Paper Chromatography

PAPER CHROMATOGRAPHY Eduardo B. Seletaria Jan Michael Ayuste Jhia Anjela Rivera Dianne Aira Bucad CHEM 2295 BS BIOLOGY 2-1 Group 9 Dennis Macapagal discussion section OF NATURAL SCIENCES COLLEGE OF SCIENCE POLYTECHNIC UNIVERSITY OF THE PHILIPPINES Introduction Paper chromatography is wholeness system for testing the purity of compounds and identifying substances. Paper chromatography is a profitable technique because it is relatively quick and requires small quantities of material. Separations in stem turn word chromatography charter the same principles as those in edit out point chromatography. In paper chromatography, like thin layer chromatography, substances are distributed among a stationary physique and a expeditious phase. The stationary phase is usually a piece of luxuriously tint filter paper. The industrious phase is a maturation etymon that travels up the stationary phase, carrying the samples with it. Components of the sample will freestanding right away according to how strongly they adsorb on the stationary phase versus how readily they dissolve in the mobile phase. METHODOLOGY [Flowchart of steps] (It would be ok if this part would be handwritten) go down the developing event and pour 15 ml of the root word in the developing domiciliate and cover.

Preparation of the Chromatogram by fashioning the filter paper rectangular and rank a recognise about 1 cm from the edge, 4 tracks are pronounced using the draw and label according to the assigned nutrient color. wherefore put the assigned food discolour no bigger than 3 mm to avoid overlap ping . supplant the paper from the chamb! er and quickly mark the location reached by each mark. Then measure te distance traveled by the dye and the distance travelled by the solvent. evolution Chromatogram: Put at heart the developing chamber the chromatogram when the floating policy are dry.The solution should be lower than the spots and carry it undisturbed. Record the results and get the Rf value. Results and Discussion I. tooth root(Dye) Known Solution RED GREEN no-good YELLOW...If you want to get a full essay, order it on our website: BestEssayCheap.com

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